Probiotic uses glycerol to kill ‘superbug’ in laboratory

By Tim Cutcliffe contact

- Last updated on GMT

© iStock
© iStock

Related tags: L. reuteri, Clostridium difficile

A species of the probiotic genus Lactobacillus may selectively kill an antibiotic resistant bacterium, according to a study in Infection and Immunity.

The addition of glycerol to Lactobacillus reuteri ​converted it into the antimicrobial compound reuterin, which preferentially targeted the pathogenic Clostridium difficile​, found the research team from Baylor College of Medicine (BCM), Houston.

The L. reuteri-​derived reuterin killed C. difficile, ​often described as a superbug, without affecting the 'good' bacteria when tested in a mini-bioreactor model. The effect was strain-specific and the reuterin was as effective in inhibiting C. difficile ​growth as the conventional antibiotic vancomycin.

“Using the mini-bioreactors model we showed that L. reuteri reduced the burden of C. difficile infection in a complex gut community,” ​commented Professor Robert Britton from BCM. “To achieve its beneficial effect, L. reuteri requires glycerol and converts it into the antimicrobial reuterin.”

Reuterin is generally known as a broad-spectrum antibiotic; it affects the growth of a wide variety of bacteria when they are tested individually in the lab.

However, to the surprise of researchers, reuterin did not have this effect in the mini-bioreactor where multiple bacterial species were present. Thus beneficial bacteria were left unharmed, unlike when conventional antibiotics are used to treat C. difficile.

“I expected reuterin to have an antibacterial effect on several different types of bacteria in the community, but it only affected C. difficile and not the good bacteria, which was exciting because it has major implications for a preventative strategy,” ​said first author Dr. Jennifer Spinler.

Significance

The findings are significant because they may enable prophylactic treatment with probiotics before taking antibiotics, which are known to disrupt ‘good’ bacteria in the gut.

Co-administering the L.reuteri ​/ glycerol would maintain the beneficial species, while preventing the growth of C. difficile​. If effective, any future treatment could have multiple benefits including shorter hospital stays, fewer cases of antibiotic induced diarrhoea and lower incidence of recurrent C.difficile ​infections.

The use of next-generation probiotics such as L.reuteri ​might also prove to bea new weapon in the fight against antibiotic resistance.

Strain-specific effect

The reuterin production capability of L. reuteri ​is strain-specific and is dependent upon an active pocR ​gene (responsible for regulating cobalmin synthesis and propanediol degradation).

The researchers identified two notable bacterial strains capable of producing reuterin in the presence of glycerol: L. reuteri ​17938 and6475. Strain 17938 produced three times as much reuterin as 6475.

Previous studies had established that the parent strain of L. reuteri ​17938 was instrinically resistant to several antibiotics including vancomycin and metronidazole. In order to comply with recommendations from the EU PROSAFE Project​ that probiotic strains should not possess antibiotic resistance traits, earlier experiments by other researchers removed the genes responsible for antibiotic resistance, resulting in the daughter strain 17938.

The future

“Although these results are too preliminary to be translated directly into human therapy, they provide a foundation upon which to further develop treatments​,” said co-author Dr. Jennifer Auchtung.

Human studies are currently in progress to determine whether co-administration of L. reuteri​ and glycerol can be used to treat the ‘superbug’ in vivo. ​The researchers are optimistic that the treatment could prove to be “a front-runner for next-generation probiotics as adjunct therapy in C. difficile infection prevention”.

 

Source:  Infection and Immunity

Volume 85, issue 8. Published online ahead of print.  doi: 10.1128/IAI.00303-17

“Next-generation probiotics targeting Clostridium difficile through precursor-directed antimicrobial biosynthesis”

Authors:  Jennifer K Spinler, Jennifer Auchtung, Robert A. Britton et al

Related news

Related products

show more

Innovative colic solutions

Innovative colic solutions

Recorded the 07-Jul-2022 | Webinar

Colic is one of the most frequent reasons for visits to the pediatrician and can often lead to parental and infant exhaustion. As a functional gastrointestinal...

Register for free

RED ORANGE COMPLEX™solution for BEAUTY FROM WITHIN

RED ORANGE COMPLEX™solution for BEAUTY FROM WITHIN

BIONAP BIOACTIVE NATURAL PRODUCTS | 07-Jun-2022 | Technical / White Paper

The intake of 100 mg/day of the standardized Red Orange Complex extract containing anthocyanins, hydroxycinnamic acids, flavanones, and ascorbic acid is...

Happier with indication-specific probiotics

Happier with indication-specific probiotics

Winclove Probiotics | 06-Jun-2022 | Product Brochure

From the very founding of Winclove Probiotics, our aim to help people, society, and the world has been an essential part of our business DNA.

Probiotical's choice for an active lifestyle

Probiotical's choice for an active lifestyle

PROBIOTICAL | 26-May-2022 | Technical / White Paper

Gastrointestinal problems, stress and mood changes, poor sleep and a weak immune system can interfere with the daily training routine in people pursuing...

Related suppliers

Follow us

Products

View more

Webinars